Preparation of Carbon Nanotube Reinforced Gelatin-Chitosan- Hydroxyapatite Biocomposite for Bone Tissue Engineering by Tanjina Islam, Khandaker S Salem, Shanta Biswas, Papia Haque, Sunzida H Rimu and Mohammed Mizanur Rahman in Open Access Journal of Biomedical Engineering and Biosciences (OAJBEB) in Lupine Publishers
Uniform and highly stable dispersion
of multi wall carbon nanotube (MWCNT) in aqueous solution using surfactant
sodium dodecyl sulphate was prepared by physical adsorption of surfactant
molecules on to the nanotubes which overcomes the van der Waals forces and
prevents aggregation between individual nanotubes. Chemically functionalized
MWCNT dispersion was also prepared by mixed acids using (3:1) (H2SO4: HNO3)
that was obtained to be less cytotoxic than the physically functionalized MWCNT
dispersion. Chemically functionalized MWCNT-Hydroxyapatite
(HAp)-Chitosan-Gelatin composite scaffold samples were prepared by solution
casting method after optimizing the cytotoxic effect results. The effect of
varying content of MWCNTs on the physico-mechanical, thermal, morphological
properties of the nanocomposites was evaluated. The chemical change of
composite with varied MWCNT content was studied using FTIR and morphological
characterization was done by SEM, where Porous structure was observed on the composites,
which is supposed to be required as criteria of a bone scaffold to grow bone
tissues. Compressive Strength (CS) of composite scaffold increased by 95.82%
with increase of MWCNT content (from 0.1% to 0.25%). The thermal characterization
of nano-composites was done by TGA and DSC and it was found that the
nanocomposite containing 0.25% MWCNTs showed highest thermal stability. TGA of
nanocomposites containing 0.25% MWCNT increased by 363.16% in comparison to
that
of nanocomposites containing 0.1%
MWCNTs. However, due to highest cytotoxicity of anocomposite having 0.25% CNTs,
0.1% composition was considered the best.
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