Ethanol Neogenesis in Stored Blood by Komal Singh in Peer Reviewed Journal of Forensic & Genetic Sciences (PRJFGS) in Lupine Publishers.
Abstract
During the investigation of accidental cases, blood
samples obtained from accidental victim are submitted to forensic science
laboratory for toxicological analysis. All through, ethanol analysis is
performed on almost all cases of blood samples. The main problem is determining
alcohol concentration in these cases is changes in Blood Alcohol Concentration
(BAC) that can occur due to neo formation of ethanol. Blood samples stored under
in sufficient conditions or for longer times and body fluids have undergone
petrifaction often contains certain amount of volatile compounds. In the light
of possibility that, neo formation of ethanol in improperly stored blood might
give rise to an incorrect estimation of BAC. With this in mind, to study the
effect of preservative and temperature on the formation and concentration of
ethanol in blood samples, we estimated the ethanol neo genesis in stored and
normal blood in different time period (0, 7, 14, 21, 28, 35 and 42 days). Head
Space-Gas Chromatography instrument used to measure the level of ethanol in
blood sample. We found that neo formation of ethanol was observed in normal
blood sample. There was no any neo formation observed in blood sample with
preservative and blood stored at 4 °C. Our study is supporting the studies
which suggest that storage condition will affect the neo formation of ethanol
in blood samples.
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