Induction of Systemic Acquired Resistance in Papaya by Foliar Application of HrpN Recombinant Protein for Increased Resistance against Papaya Dieback Pathogen by Norliza Abu Bakar* in Current Investigations in Agriculture and Current Research in Lupine Publishers
Plants are continuously exposed to undesirable pest and pathogen
threats. In response, plants have developed numerous
mechanisms to protect themselves against the pathogens. Systemic
acquired resistance (SAR) is an inducible disease resistance
response in plant species. It is found in a large range of plant species
including papaya and characterized by broad spectrum disease
control and an associated coordinated expression of a set of
pathogenesis related (PR) genes and proteins which are also known
as SAR markers. Expression and purification of HrpN from Erwinia
mallotivora, the causal agent of papaya dieback, was carried
out. In this report, HrpN recombinant protein was tested and
characterized for its effect and potential as elicitor that can increase
papaya defence against E. mallotivora through the activation of SAR
mechanism. Based on disease severity analysis, control plants
which were untreated, showed faster disease infection rate and severity
when compared to the recombinant protein treated plants.
Increased resistance towards the papaya dieback pathogen was shown to be
associated with increased expression of selected plant
defined genes using quantitative Real Time analysis which were observed
after the papaya was sprayed with the recombinant HrpN
protein. Based on physiological and molecular analysis, the selected
protein has induced SAR; increased selected SAR associated
defence gene expression and increased the papaya resistance against the
papaya dieback pathogen.
No comments:
Post a Comment
Note: only a member of this blog may post a comment.